ARG22980

anti-CD16 antibody [G7] (PE)

Datasheet

参考文献1

订购信息

包装

价格 (USD)

50 tests

425

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [G7] recognizes CD16 Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
反应物种	Pig
应用	FACS
宿主	Mouse
克隆	Monoclonal
克隆号	G7
同位型	IgG1
靶点名称	CD16
抗原物种	Pig
抗原	Porcine peripheral blood leucocytes
偶联标记	PE
別名	FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

应用说明

应用建议

应用	推荐稀释比
FACS	Neat

应用说明

FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose
抗菌剂	0.09% Sodium azide
稳定剂	1% BSA and 5% Sucrose
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	FCGR3A
全名	Fc fragment of IgG receptor IIIa
背景介绍	This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能	Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
产品亮点	Related products: CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tumor-Infiltrating Lymphocytes (TILs)
研究领域	Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
预测分子量	29 kDa
翻译后修饰	Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. The soluble form is produced by a proteolytic cleavage.

检测图片 (3) Click the Picture to Zoom In

ARG22980 anti-CD16 antibody [G7] (PE) FACS image

Flow Cytometry: Porcine peripheral blood granulocytes stained with ARG22980 anti-CD16 antibody [G7] (PE).
ARG22980 anti-CD16 antibody [G7] (PE) FACS image

Flow Cytometry: Figure A. FITC conjugated mouse anti porcine CD45 and PE conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti porcine CD45 and ARG22980 anti-CD16 antibody [G7] (PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
ARG22980 anti-CD16 antibody [G7] (PE) FACS image

Flow Cytometry: Figure A. ARG22980 anti-CD16 antibody [G7] (PE) and FITC conjugated mouse IgG1 isotype control. Figure B. ARG22980 anti-CD16 antibody [G7] (PE) and FITC conjugated mouse anti porcine CD45. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.