ARG65461

anti-CD161 antibody [HP-3G10]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
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0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [HP-3G10] recognizes CD161
反应物种	Hu, NHuPrm
应用	CyTOF®-candidate, FACS, WB
特异性	The clone HP-3G10 recognizes CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer.
宿主	Mouse
克隆	Monoclonal
克隆号	HP-3G10
同位型	IgG1
靶点名称	CD161
抗原物种	Human
抗原	human NK cells
偶联标记	Un-conjugated
別名	CLEC5B; CD antigen CD161; CD161; NKR-P1; NKR-P1A; Killer cell lectin-like receptor subfamily B member 1; NKRP1A; NKR; HNKR-P1a; Natural killer cell surface protein P1A; C-type lectin domain family 5 member B; hNKR-P1A

应用说明

应用建议

应用	推荐稀释比
CyTOF®-candidate	Assay-dependent
FACS	1 - 4 µg/ml
WB	Assay-dependent

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3820 Human KLRB1 Swiss-port # Q12918 Human Killer cell lectin-like receptor subfamily B member 1
基因名称	KLRB1
全名	killer cell lectin-like receptor subfamily B, member 1
背景介绍	CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on a majority of NK cells, NKT cells, and e.g. Th17 cells and CD3+ thymocytes. Although rat CD161 has three isoforms (a, b, c), the human CD161 is expressed as one isoform.
生物功能	Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. [UniProt]
产品亮点	Related products: CD161 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	25 kDa
翻译后修饰	N-glycosylated. Contains sialic acid residues.

检测图片 (2) Click the Picture to Zoom In

ARG65461 anti-CD161 antibody [HP-3G10] FACS image

Flow Cytometry: Human peripheral blood cells stained with ARG65461 anti-CD161 antibody [HP-3G10] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG65461 anti-CD161 antibody [HP-3G10] FACS image

Flow Cytometry: Separation of human CD161 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG65461 anti-CD161 antibody [HP-3G10] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.