ARG42302
anti-CD169 / Siglec 1 antibody [7-239] (APC)
anti-CD169 / Siglec 1 antibody [7-239] (APC) for Flow cytometry and Human
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [7-239] recognizes CD169 / Siglec 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 7-239 |
| 同位型 | IgG1 |
| 靶点名称 | CD169 / Siglec 1 |
| 抗原物种 | Human |
| 抗原 | Human rhinovirus 14-infected monocyte-derived dendritic cells. |
| 偶联标记 | APC |
| 別名 | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SIGLEC1 |
| 全名 | sialic acid binding Ig-like lectin 1, sialoadhesin |
| 背景介绍 | This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. [UniProt] |
| 预测分子量 | 183 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension.
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension and ARG62720 anti-CD11c antibody [BU15] (FITC) at 20 µl / 100 µl of peripheral whole blood.
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ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) FACS image
Flow Cytometry: Separation of Human CD169 positive CD11c positive cells (red-filled) from CD169 negative CD11c negative cells (black-dashed). Human TNF alpha and INF gamma stimulated peripheral blood mononuclear cells stained with ARG42302 anti-CD169 / Siglec 1 antibody [7-239] (APC) at 10 µl / 10^6 cells in 100 µl of cell suspension.
