ARG65404
anti-CD169 / Siglec 1 antibody [7-239]
anti-CD169 / Siglec 1 antibody [7-239] for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Mouse Monoclonal antibody [7-239] recognizes CD169 / Siglec 1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-Fr, IP, WB |
| 特异性 | The mouse monoclonal antibody 7239 recognizes CD169 (sialoadhesin, Siglec1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 7-239 |
| 同位型 | IgG1 |
| 靶点名称 | CD169 / Siglec 1 |
| 抗原物种 | Human |
| 抗原 | human rhinovirus 14-infected monocyte-derived dendritic cells |
| 偶联标记 | Un-conjugated |
| 別名 | CD169; Siglec-1; dJ1009E24.1; Sialic acid-binding Ig-like lectin 1; SIGLEC-1; CD antigen CD169; SN; Sialoadhesin |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Inhibition of erythrocyte rosetting with cells expressing CD169. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SIGLEC1 |
| 全名 | sialic acid binding Ig-like lectin 1, sialoadhesin |
| 背景介绍 | CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses. |
| 生物功能 | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. [UniProt] |
| 研究领域 | Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 183 kDa |
