ARG54289
anti-CD178 / Fas Ligand antibody [NOK-1] (PE)
anti-CD178 / Fas Ligand antibody [NOK-1] (PE) for Flow cytometry and Human
Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [NOK-1] recognizes CD178 / Fas Ligand |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone NOK-1 recognizes CD178 / Fas-L, an approximately 40 kDa transmembrane glycoprotein expressed on neutrophils, monocytes, and activated T and NK cells. HCDM Workshop: VII 70322 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | NOK-1 |
| 同位型 | IgG1 |
| 靶点名称 | CD178 / Fas Ligand |
| 抗原物种 | Human |
| 抗原 | L5178Y mouse T lymphoma cells expressing recombinant human CD178 |
| 偶联标记 | PE |
| 別名 | FasL ICD; SPPL2A-processed FasL form; Apoptosis antigen ligand; CD95 ligand; CD178; Fas antigen ligand; CD95-L; Receptor-binding FasL ectodomain; FasL; SPA; TNFSF6; CD95L; FASL; Fas ligand; APTL; APT1LG1; ALPS1B; sFasL; Soluble Fas ligand; Tumor necrosis factor ligand superfamily member 6; APL; CD antigen CD178 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P48023 Human Tumor necrosis factor ligand superfamily member 6 |
|---|---|
| 基因名称 | FASLG |
| 全名 | Fas ligand (TNF superfamily, member 6) |
| 背景介绍 | CD178 / Fas-L (Fas ligand, CD95L), a member of TNF family transmembrane glycoproteins, is responsible for induction of apoptosis in cells containing its receptor CD95 / Fas. The CD178-mediated apoptosis pathway has been implicated in peripheral tolerance, tissue pathology, and maintenance of the immune privileged sites. Defects in this interaction may be related to some cases of systemic lupus erythematosus (SLE). CD178 was also described as a co-stimulatory receptor for T-cell activation in mice in vivo. |
| 生物功能 | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody; Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells. N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260). Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization. Monoubiquitinated. |
检测图片 (1) Click the Picture to Zoom In
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ARG54289 anti-CD178 / Fas Ligand antibody [NOK-1] (PE) FACS image
Flow Cytometry: Separation of CD178 transfected L5178Y cells (red) from non-transfected L5178Y cells (black-dashed). Cells were stained with ARG54289 anti-CD178 / Fas Ligand antibody [NOK-1] (PE) at 1 µg/ml dilution.
