ARG62754

anti-CD18 / LFA1 beta antibody [IVA35]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Mouse Monoclonal antibody [IVA35] recognizes CD18 / LFA1 beta
反应物种	Bov
应用	FACS, ICC/IF, IP
特异性	The clone IVA35 reacts with CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
宿主	Mouse
克隆	Monoclonal
克隆号	IVA35
同位型	IgG1
靶点名称	CD18 / LFA1 beta
抗原	Bovine leukocytes.
偶联标记	Un-conjugated
別名	MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 4 µg/ml
ICC/IF	Assay-dependent
IP	Assay-dependent

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from ascites by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 281877 Bovine ITGB2 Swiss-port # P32592 Bovine Integrin beta-2
基因名称	ITGB2
全名	integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
背景介绍	This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
生物功能	Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (By similarity). Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
预测分子量	85 kDa
翻译后修饰	Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335).

检测图片 (3) Click the Picture to Zoom In

ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] ICC/IF image

Immunofluorescence: Acetone / methanol-fixed Bovine peripheral blood (lymphocyte-enriched suspension) stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] (green), DAPI (blue) for nuclear staining.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

Flow Cytometry: Separation of stained bovine lymphocytes (red-filled) from unstained bovine lymphocytes (black-dashed). Cells were stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] FACS image

Flow Cytometry: Bovine peripheral whole blood stained with ARG62754 anti-CD18 / LFA1 beta antibody [IVA35] at 10 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.