ARG62757
anti-CD18 / LFA1 beta antibody [M18 / 2]
anti-CD18 / LFA1 beta antibody [M18 / 2] for Flow cytometry,Functional study,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rat Monoclonal antibody [M18/2] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Ms |
| 不反应物种 | Dog |
| 应用 | FACS, FuncSt, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | M18/2 |
| 同位型 | IgG2a |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原物种 | Mouse |
| 抗原 | Murine cytotoxic T cell glycoproteins |
| 偶联标记 | Un-conjugated |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Blocking or stimulation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Itgb2 |
| 全名 | integrin beta 2 |
| 背景介绍 | CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
检测图片 (2) Click the Picture to Zoom In
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ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] FACS image
Flow Cytometry: Murine splenocytes stained with ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] at 16 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
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ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] FACS image
Flow Cytometry: Separation of unstained (black-dashed) and stained (red-filled) murine myeloid cells from murine splenocyte suspension. Cells were stained with ARG62757 anti-CD18 / LFA1 beta antibody [M18 / 2] at 16 µg/ml dilution, followed by APC-conjugated Donkey anti-Rat antibody.
克隆号文献
Neutrophil-selective CD18 silencing using RNA interference in vivo.
Manifestations of inflammatory arthritis are critically dependent on LFA-1.
CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes.
Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization.
Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1.
