ARG53780
anti-CD18 / LFA1 beta antibody [MEM-48] (PE)
anti-CD18 / LFA1 beta antibody [MEM-48] (PE) for Flow cytometry and Human
Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [MEM-48] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Dog |
| 应用 | FACS |
| 特异性 | The clone MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-48 |
| 同位型 | IgG1 |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原 | Leukocytes of a patient suffering from a LGL-type leukemia. |
| 偶联标记 | PE |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB2 |
| 全名 | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| 背景介绍 | CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
检测图片 (1) Click the Picture to Zoom In
-
ARG53780 anti-CD18 / LFA1 beta antibody [MEM-48] (PE) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG53780 anti-CD18 / LFA1 beta antibody [MEM-48] (PE).
Cells in the granulocyte gate were used for analysis.
克隆号文献
Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
ICC/IF /
Analysis on the relevance of asthma susceptibility with the alteration of integrin β 4 expression.
WB / Human
Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow.
NKp80 defines and stimulates a reactive subset of CD8 T cells.
Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation.
Human leukocytes contain a large pool of free forms of CD18.
