ARG22447
anti-CD18 / LFA1 beta antibody [PNK-I]
anti-CD18 / LFA1 beta antibody [PNK-I] for Flow cytometry,Functional study,Immunoprecipitation and Camel,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [PNK-I] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Camel, Pig |
| 应用 | FACS, FuncSt, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PNK-I |
| 同位型 | IgG1 |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原物种 | Pig |
| 抗原 | Porcine large granular lymphocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FuncSt: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB2 |
| 全名 | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| 背景介绍 | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
检测图片 (2) Click the Picture to Zoom In
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ARG22447 anti-CD18 / LFA1 beta antibody [PNK-I] FACS image
Flow Cytometry: Porcine peripheral blood granulocytes stained with ARG22447 anti-CD18 / LFA1 beta antibody [PNK-I].
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ARG22447 anti-CD18 / LFA1 beta antibody [PNK-I] FACS image
Flow Cytometry: Peripheral blood lymphocytes stained with ARG22447 anti-CD18 / LFA1 beta antibody [PNK-I] followed by Goat anti Mouse IgG (FITC).
