ARG23053
anti-CD18 / LFA1 beta antibody [YFC118.3] (FITC)
anti-CD18 / LFA1 beta antibody [YFC118.3] (FITC) for Flow cytometry and Human,Dog,Guinea pig
概述
| 产品描述 | FITC-conjugated Rat Monoclonal antibody [YFC118.3] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Hu, Dog, Gpig |
| 应用 | FACS |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | YFC118.3 |
| 同位型 | IgG2b |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原物种 | Human |
| 抗原 | Human neutrophils. |
| 偶联标记 | FITC |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide and 1% BSA |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA |
| 浓度 | 0.1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB2 |
| 全名 | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| 背景介绍 | This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
检测图片 (1) Click the Picture to Zoom In
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ARG23053 anti-CD18 / LFA1 beta antibody [YFC118.3] (FITC) FACS image
Flow Cytometry: Figure A. RPE conjugated Mouse anti Human CD19 and FITC conjugated Rat IgG2b isotype control. Figure B. RPE conjugated Mouse anti Human CD19 and ARG23053 anti-CD18 / LFA1 beta antibody [YFC118.3] (FITC). All experiments performed on Human peripheral blood lymphocytes.
