ARG54246
anti-CD180 / RP105 antibody [G28-8] (APC)
anti-CD180 / RP105 antibody [G28-8] (APC) for Flow cytometry and Human
Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [G28-8] recognizes CD180 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | G28-8 |
| 同位型 | IgG1 |
| 靶点名称 | CD180 / RP105 |
| 抗原物种 | Human |
| 抗原 | Human tonsillar B cells |
| 偶联标记 | APC |
| 別名 | LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD180 |
| 全名 | CD180 molecule |
| 背景介绍 | CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size. |
| 生物功能 | May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt] |
| 研究领域 | Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 74 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG54246 anti-CD180 / RP105 antibody [G28-8] (APC) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG54246 anti-CD180 / RP105 antibody [G28-8] (APC) and anti-CD19 antibody (PE).
克隆号文献
Caspase activity is required for stimulated B lymphocytes to enter the cell cycle.
Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation.
