ARG65445
anti-CD180 / RP105 antibody [G28-8]
anti-CD180 / RP105 antibody [G28-8] for Flow cytometry,Functional study,Immunoprecipitation and Human
Immune System antibody; Microbiology and Infectious Disease antibody
概述
| 产品描述 | Mouse Monoclonal antibody [G28-8] recognizes CD180 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IP |
| 特异性 | The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | G28-8 |
| 同位型 | IgG1 |
| 靶点名称 | CD180 / RP105 |
| 抗原物种 | Human |
| 抗原 | Human tonsillar B cells |
| 偶联标记 | Un-conjugated |
| 別名 | LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Functional studies: Induction of B cell proliferation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD180 |
| 全名 | CD180 molecule |
| 背景介绍 | CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size. |
| 生物功能 | May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt] |
| 研究领域 | Immune System antibody; Microbiology and Infectious Disease antibody |
| 预测分子量 | 74 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65445 anti-CD180 / RP105 antibody [G28-8] FACS image
Flow Cytometry: Human peripheral blood stained with ARG65445 anti-CD180 / RP105 antibody [G28-8] at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65445 anti-CD180 / RP105 antibody [G28-8] FACS image
Flow Cytometry: Separation of human CD180 positive lymphocytes (red-filled) from CD180 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG65445 anti-CD180 / RP105 antibody [G28-8] at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
Caspase activity is required for stimulated B lymphocytes to enter the cell cycle.
Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation.
