ARG42261
anti-CD180 / RP105 antibody [G28-8] (low endotoxin)
anti-CD180 / RP105 antibody [G28-8] (low endotoxin) for Flow cytometry,Functional study,Immunoprecipitation and Human
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [G28-8] recognizes CD180 / RP105 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IP |
| 特异性 | The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | G28-8 |
| 同位型 | IgG1, kappa |
| 靶点名称 | CD180 / RP105 |
| 抗原物种 | Human |
| 抗原 | Human tonsillar B cells. |
| 偶联标记 | Un-conjugated |
| 別名 | LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD180 |
| 全名 | CD180 molecule |
| 背景介绍 | CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. [provided by RefSeq, Jul 2008] |
| 生物功能 | May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 74 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42261 anti-CD180 / RP105 antibody [G28-8] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42261 anti-CD180 / RP105 antibody [G28-8] (low endotoxin) at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42261 anti-CD180 / RP105 antibody [G28-8] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD180 positive lymphocytes (red-filled) from CD180 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42261 anti-CD180 / RP105 antibody [G28-8] (low endotoxin) at 6 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
