ARG65383
anti-CD20 antibody [2H7]
anti-CD20 antibody [2H7] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Human,Primates
Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [2H7] recognizes CD20 |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | CyTOF®-candidate, FACS, IHC-Fr, IP |
| 特异性 | The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 3337 kDa nonglycosylated membrane receptor with four transmembrane domains, expressed on preB lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2H7 |
| 同位型 | IgG2b |
| 靶点名称 | CD20 |
| 抗原物种 | Human |
| 抗原 | Human tonsillar B cells |
| 偶联标记 | Un-conjugated |
| 別名 | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MS4A1 |
| 全名 | membrane-spanning 4-domains, subfamily A, member 1 |
| 背景介绍 | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| 产品亮点 | Related products: CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylated. Might be functionally regulated by protein kinase(s). |
克隆号文献
CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells.
Novel follicular dendritic cell molecule, 8D6, collaborates with CD44 in supporting lymphomagenesis by a Burkitt lymphoma cell line, L3055.
Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment.
Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.
