ARG62767
anti-CD20 antibody [MEM-97]
anti-CD20 antibody [MEM-97] for Flow cytometry,Immunoprecipitation and Human,Bovine,Pig
Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-97] recognizes CD20 |
|---|---|
| 反应物种 | Hu, Bov, Pig |
| 应用 | FACS, IP |
| 特异性 | The clone MEM-97 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. HLDA V; WS Code B CD20.9 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-97 |
| 同位型 | IgG1 |
| 靶点名称 | CD20 |
| 抗原物种 | Human |
| 抗原 | Raji human Burkitt's lymphoma cell line |
| 偶联标记 | Un-conjugated |
| 別名 | Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MS4A1 |
| 全名 | membrane-spanning 4-domains, subfamily A, member 1 |
| 背景介绍 | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008] |
| 生物功能 | CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30308 Immature B Cell Marker Antibody Panel (CD19, CD20, CD22, IgM Fc) (FACS) Related products: CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylated. Might be functionally regulated by protein kinase(s). |
检测图片 (2) Click the Picture to Zoom In
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ARG62767 anti-CD20 antibody [MEM-97] IP image
Immunoprecipitation: Immunoprecipitation of human CD20 from the whole cell lysate of Raji cells.
1) original lysate of Raji cells, and 2) same cells immunoprecipitate by ARG62767 anti-CD20 antibody [MEM-97].
Western blot was stained with anti-CD20 rabbit polyclonal antibody. . -
ARG62767 anti-CD20 antibody [MEM-97] FACS image
Flow Cytometry: Human, canine and porcine B lymphocytes double stained with ARG62767 anti-CD20 antibody [MEM-97] and anti-CD79a antibody [HM57].
克隆号文献
A robust approach to enhance tumor-selective accumulation of nanoparticles.
Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes.
FACS / Human
Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY.
