ARG62778
anti-CD222 / IGF2R antibody [MEM-238] (FITC)
anti-CD222 / IGF2R antibody [MEM-238] (FITC) for Flow cytometry and Human,Primates
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [MEM-238] recognizes CD222 / IGF2R |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS |
| 特异性 | The clone MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-238 |
| 同位型 | IgG1 |
| 靶点名称 | CD222 / IGF2R |
| 抗原 | Recombinant Vaccinia virus encoding CD222. |
| 偶联标记 | FITC |
| 別名 | CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor |
|---|---|
| 基因名称 | IGF2R |
| 全名 | insulin-like growth factor 2 receptor |
| 背景介绍 | CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria. |
| 生物功能 | Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt] |
| 研究领域 | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 274 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62778 anti-CD222 / IGF2R antibody [MEM-238] (FITC) FACS image
Flow Cytometry: Human peripheral blood stained with ARG62778 anti-CD222 / IGF2R antibody [MEM-238] (FITC) (red) or Isotype control antibody (blue).
克隆号文献
Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system.
ICC/IF / Human
The C proteins of human parainfluenza virus type 1 block IFN signaling by binding and retaining Stat1 in perinuclear aggregates at the late endosome.
ICC/IF /
Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions.
FACS / Human
The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.
ICC/IF / Human
Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression.
Inhib / Human
Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation.
IP / Mouse
