ARG62782
anti-CD25 antibody [MEM-181] (Biotin)
anti-CD25 antibody [MEM-181] (Biotin) for Flow cytometry and Human
Immune System antibody; Pre-B Cell Marker antibody
概述
| 产品描述 | Biotin-conjugated Mouse Monoclonal antibody [MEM-181] recognizes CD25 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. HLDA VI; WS Code NL N-L024 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-181 |
| 同位型 | IgG1 |
| 靶点名称 | CD25 |
| 抗原 | PHA-activated peripheral blood leucocytes |
| 偶联标记 | Biotin |
| 別名 | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha |
|---|---|
| 基因名称 | IL2RA |
| 全名 | interleukin 2 receptor, alpha |
| 背景介绍 | CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas. |
| 生物功能 | Receptor for interleukin-2. [UniProt] |
| 研究领域 | Immune System antibody; Pre-B Cell Marker antibody |
| 预测分子量 | 31 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62782 anti-CD25 antibody [MEM-181] (Biotin) FACS image
Flow Cytometry: PHA-stimulated (3 days) Human PBMC stained with ARG62782 anti-CD25 antibody [MEM-181] (Biotin), followed by Streptavidin (APC).
克隆号文献
Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.
ICC/IF / Human
Different motifs regulate trafficking of SorCS1 isoforms.
ICC/IF / Mouse
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
