ARG56597
anti-CD254 / RANKL antibody [2.1_3C12-2D11]
anti-CD254 / RANKL antibody [2.1_3C12-2D11] for ELISA and Human
概述
产品描述 | Mouse Monoclonal antibody [2.1_3C12-2D11] recognizes CD254 / RANKL |
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反应物种 | Hu |
应用 | ELISA |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 2.1_3C12-2D11 |
同位型 | IgG1, kappa |
靶点名称 | CD254 / RANKL |
抗原物种 | Human |
抗原 | E.coli derived Recombinant Human CD254 / RANKL. (MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID) |
偶联标记 | Un-conjugated |
別名 | TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254 |
应用说明
应用建议 |
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应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.2) |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 |
Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11 |
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基因名称 | TNFSF11 |
全名 | tumor necrosis factor (ligand) superfamily, member 11 |
背景介绍 | This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008] |
生物功能 | Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt] |
预测分子量 | 35 kDa |
翻译后修饰 | The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. |
检测图片 (1) Click the Picture to Zoom In
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ARG56597 anti-CD254 / RANKL antibody [2.1_3C12-2D11] standard curve image
Sandwich ELISA: ARG56597 anti-CD254 / RANKL antibody [2.1_3C12-2D11] as a capture antibody at 1.0 - 2.0 µg/ml combined with ARG56762 anti-CD254 / RANKL antibody (Biotin) as a detection antibody at ~ 0.125 - 0.25 µg/ml. Results of a typical standard run with optical density.