ARG44258

anti-CD254 / RANKL antibody

anti-CD254 / RANKL antibody for Flow cytometry,ICC/IF and Human,Mouse

概述

产品描述 Goat Polyclonal antibody recognizes CD254 / RANKL
反应物种 Hu, Ms
应用 FACS, ICC/IF
特异性 This antibody is expected to recognise both reported isoforms
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 CD254 / RANKL
抗原物种 Human
抗原 Synthetic peptide around the internal region of Human CD254 / RANKL (DLAKRSKLEAQP)
偶联标记 Un-conjugated
別名 TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

应用说明

应用建议
应用 推荐稀释比
FACS10 µg/µl
ICC/IF10 µg/µl
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 21943 Mouse TNFSF11

GeneID: 8600 Human TNFSF11

Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11

Swiss-port # O35235 Mouse Tumor necrosis factor ligand superfamily member 11

基因名称 TNFSF11
全名 tumor necrosis factor (ligand) superfamily, member 11
背景介绍 This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
生物功能 Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]
翻译后修饰 The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

检测图片 (2) Click the Picture to Zoom In

  • ARG44258 anti-CD254 / RANKL antibody ICC/IF image

    Immunofluorescence: MCF7 stained with ARG44258 anti-CD254 / RANKL antibody at 10 µg/m dilution.

  • ARG44258 anti-CD254 / RANKL antibody FACS image

    Flow Cytometry: HeLa stained with ARG44258 anti-CD254 / RANKL antibody at 10 µg/m dilution.