ARG55101

anti-CD254 / RANKL antibody

anti-CD254 / RANKL antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Immune System antibody

概述

产品描述 Rabbit Polyclonal antibody recognizes CD254 / RANKL
反应物种 Hu, Ms, Rat
应用 ELISA, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CD254 / RANKL
抗原 Synthetic peptide within aa. 140-190 of Human CD254 / RANKL.
偶联标记 Un-conjugated
別名 TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
IHC-P5 μg/ml
WB0.5 - 2 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.02% Sodium azide
抗菌剂 0.02% Sodium azide
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 117516 Rat TNFSF11

GeneID: 21943 Mouse TNFSF11

GeneID: 8600 Human TNFSF11

基因名称 TNFSF11
全名 tumor necrosis factor (ligand) superfamily, member 11
背景介绍 anti-RANKL antibody: The receptor activator of NF-κB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
生物功能 Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]
研究领域 Cell Biology and Cellular Response antibody; Immune System antibody
预测分子量 35 kDa
翻译后修饰 The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

检测图片 (4) Click the Picture to Zoom In

  • ARG55101 anti-CD254 / RANKL antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde and block with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in citrate buffer (pH 6.0). Samples were stained with ARG55101 anti-CD254 / RANKL antibody at 5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin.

  • ARG55101 anti-CD254 / RANKL antibody WB image

    Western blot: 15 µg of A431, HeLa and K562 cell lysates stained with ARG55101 anti-CD254 / RANKL antibody at 1 µg/ml dilution and incubated at RT for 1 hour, in 5% NFDM/TBST.

  • ARG55101 anti-CD254 / RANKL antibody WB image

    Western blot: 15 µg of Mouse kidney and Mouse skin lysates stained with ARG55101 anti-CD254 / RANKL antibody at 2 µg/ml dilution and incubated at RT for 1 hour, in 5% NFDM/TBST.

  • ARG55101 anti-CD254 / RANKL antibody WB image

    Western blot: 15 µg of Rat liver lysate stained with ARG55101 anti-CD254 / RANKL antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour, in 5% NFDM/TBST.