ARG56762

anti-CD254 / RANKL antibody (Biotin)

anti-CD254 / RANKL antibody (Biotin) for ELISA,Western blot and Human

概述

产品描述 Biotin-conjugated Rabbit Polyclonal antibody recognizes CD254 / RANKL
反应物种 Hu
应用 ELISA, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CD254 / RANKL
抗原物种 Human
抗原 E.coli derived Recombinant Human CD254 / RANKL.
(MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID)
偶联标记 Biotin
別名 TRANCE; Osteoprotegerin ligand; CD254; sOdf; Receptor activator of nuclear factor kappa-B ligand; OPTB2; RANKL; OPGL; Tumor necrosis factor ligand superfamily member 11; hRANKL2; TNF-related activation-induced cytokine; Osteoclast differentiation factor; ODF; CD antigen CD254

应用说明

应用建议
应用 推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56652 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified by affinity chromatography.
缓冲液 PBS (pH 7.2)
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 8600 Human TNFSF11

Swiss-port # O14788 Human Tumor necrosis factor ligand superfamily member 11

基因名称 TNFSF11
全名 tumor necrosis factor (ligand) superfamily, member 11
背景介绍 This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]
生物功能 Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. [UniProt]
预测分子量 35 kDa
翻译后修饰 The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

检测图片 (4) Click the Picture to Zoom In

  • ARG56762 anti-CD254 / RANKL antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sRANKL stained with ARG56762 anti-CD254 / RANKL antibody (Biotin), under reducing conditions.

  • ARG56762 anti-CD254 / RANKL antibody (Biotin) standard curve image

    Direct ELISA: ARG56762 anti-CD254 / RANKL antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56762 anti-CD254 / RANKL antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human sRANKL stained with ARG56762 anti-CD254 / RANKL antibody (Biotin), under non-reducing conditions.

  • ARG56762 anti-CD254 / RANKL antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56762 anti-CD254 / RANKL antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56652 anti-CD254 / RANKL antibody as a capture antibody. Results of a typical standard run with optical density.