ARG65468
anti-CD266 / TWEAK R antibody [ITEM-4]
anti-CD266 / TWEAK R antibody [ITEM-4] for Flow cytometry,Functional study,IHC-Frozen sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [ITEM-4] recognizes CD266 / TWEAK R |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, FuncSt, IHC-Fr, WB |
| 特异性 | The clone ITEM-4 recognizes CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ITEM-4 |
| 同位型 | IgG2b |
| 靶点名称 | CD266 / TWEAK R |
| 抗原物种 | Human |
| 抗原 | human CD266-transfected P815 cells |
| 偶联标记 | Un-conjugated |
| 別名 | Tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; CD266; Fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR; TweakR; CD antigen CD266; Tweak-receptor; FN14 |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: Blocking. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9CR75 Mouse Tumor necrosis factor receptor superfamily member 12A Swiss-port # Q9NP84 Human Tumor necrosis factor receptor superfamily member 12A |
|---|---|
| 基因名称 | TNFRSF12A |
| 全名 | tumor necrosis factor receptor superfamily, member 12A |
| 背景介绍 | CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential. |
| 生物功能 | Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 14 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] FACS image
Flow Cytometry: HUVEC cells stained with ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] FACS image
Flow Cytometry: Separation of stained HUVEC cells (red-filled) from unstained HUVEC cells (black-dashed). Cells were stained with ARG65468 anti-CD266 / TWEAK R antibody [ITEM-4] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
TWEAK mediates signal transduction and differentiation of RAW264.7 cells in the absence of Fn14/TweakR. Evidence for a second TWEAK receptor.
