ARG62793
anti-CD27 antibody [LT27]
anti-CD27 antibody [LT27] for Flow cytometry and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [LT27] recognizes CD27 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone LT27 reacts with CD27 (T14), a 50-55 kDa type I transmembrane glycoprotein (member of the TNF-receptor superfamily) expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes and NK cells. HLDA V; WS Code T T-CD27.01 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LT27 |
| 同位型 | IgG2a |
| 靶点名称 | CD27 |
| 抗原物种 | Human |
| 抗原 | Human peripheral blood lymphocytes |
| 偶联标记 | Un-conjugated |
| 別名 | T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by precipitation methods and ion exchange chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD27 |
| 全名 | CD27 molecule |
| 背景介绍 | CD27 is a transmembrane 55 kDa protein of the nerve growth factor-receptor family, expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. This sCD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. |
| 生物功能 | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt] |
| 研究领域 | Cancer antibody; Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans. |
克隆号文献
Reduced expression of adhesion molecules and cell signaling receptors by chronic lymphocytic leukemia cells with 11q deletion.
