ARG65518
anti-CD271 / NGFR p75 antibody [NGFR5]
anti-CD271 / NGFR p75 antibody [NGFR5] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Ferret,Primates,Rabbit
Developmental Biology antibody; Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody [NGFR5] recognizes CD271 / NGFR p75 |
|---|---|
| 反应物种 | Hu, Cat, Frt, NHuPrm, Rb |
| 不反应物种 | Ms, Rat |
| 应用 | CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | The mouse monoclonal antibody NGFR5 (originally C34C) recognizes CD271 / NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within amino acids 1 - 160. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | NGFR5 |
| 同位型 | IgG1 |
| 靶点名称 | CD271 / NGFR p75 |
| 抗原物种 | Human |
| 抗原 | Purified CD271 protein isolated from human melanoma cell line A875 |
| 偶联标记 | Un-conjugated |
| 別名 | Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor |
应用说明
| 应用建议 |
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| 应用说明 | Immunohistochemistry (paraffin sections): Positive tissue: melanoma Application note: heat-mediated antigen retrieval * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16 |
|---|---|
| 基因名称 | NGFR |
| 全名 | nerve growth factor receptor |
| 背景介绍 | CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells. |
| 生物功能 | Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt] |
| 研究领域 | Developmental Biology antibody; Neuroscience antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | N- and O-glycosylated. O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. Phosphorylated on serine residues. |
检测图片 (3) Click the Picture to Zoom In
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ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human melanoma tissue stained with ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5].
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ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5] IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5].
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ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5] CyTOF image
CyTOF: Human brain tumor cells were stained with ARG65518 anti-CD271 / NGFR p75 antibody [NGFR5] (143Nd). Singlet cells were gated for data analysis.
克隆号文献
Induced periosteum a complex cellular scaffold for the treatment of large bone defects.
IHC-P, FACS / Human
Pathology of experimental aerosol Zaire ebolavirus infection in rhesus macaques.
The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.
ICC/IF / Human
Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.
IHC / Human
Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271.
IHC-P / Human
Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice.
IHC / Mouse
Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line.
WB / Human
Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells.
FACS / Human
