ARG65473
anti-CD28 antibody [JJ319] (PE)
anti-CD28 antibody [JJ319] (PE) for Flow cytometry and Rat
Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [JJ319] recognizes CD28 |
|---|---|
| 反应物种 | Rat |
| 应用 | FACS |
| 特异性 | The clone JJ319 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells. _x000D_ |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | JJ319 |
| 同位型 | IgG1 |
| 靶点名称 | CD28 |
| 抗原物种 | Rat |
| 抗原 | Mouse A20J B lymphoma cells transfected with rat CD28 |
| 偶联标记 | PE |
| 別名 | Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 0.5 mg/ml |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P31042 Rat T-cell-specific surface glycoprotein CD28 |
|---|---|
| 基因名称 | Cd28 |
| 全名 | Cd28 molecule |
| 背景介绍 | CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. Its analog CTLA-4, expressed on the surface of activated T cells, has higher affinity to CD80 and CD86 than CD28 and downregulates T cell activity. |
| 生物功能 | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | CD40LG induces tyrosine phosphorylation of isoform 3. |
克隆号文献
Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester.
