ARG22952
anti-CD31 antibody [LCI-4] (APC)
anti-CD31 antibody [LCI-4] (APC) for Flow cytometry and Human,Pig
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [LCI-4] recognizes CD31 Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmambrane region as demonstrated by human CD31 domain deletion mutant protein binding studies (Nasu et al.1999). Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999). |
|---|---|
| 反应物种 | Hu, Pig |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LCI-4 |
| 同位型 | IgG1 |
| 靶点名称 | CD31 |
| 抗原物种 | Pig |
| 抗原 | Porcine CD31/human IgGFc fusion protein. |
| 偶联标记 | APC |
| 別名 | EndoCAM; CD31/EndoCAM; PECAM-1; CD31; PECA1; CD antigen CD31; GPIIA'; endoCAM; Platelet endothelial cell adhesion molecule |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P16284 Human Platelet endothelial cell adhesion molecule Swiss-port # Q95242 Pig Platelet endothelial cell adhesion molecule |
|---|---|
| 基因名称 | PECAM1 |
| 全名 | platelet/endothelial cell adhesion molecule 1 |
| 背景介绍 | CD31 protein is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010] |
| 生物功能 | CD31 is a cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis. Isoform Delta15: Does not protect against apoptosis. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Signaling Transduction antibody; Endothelial Cell Marker antibody; Microvascular Density Study antibody |
| 预测分子量 | 83 kDa |
| 翻译后修饰 | Phosphorylated on Ser and Tyr residues after cellular activation. Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts. |
检测图片 (1) Click the Picture to Zoom In
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ARG22952 anti-CD31 antibody [LCI-4] (APC) FACS image
Flow Cytometry: Figure A. ARG22952 anti-CD31 antibody [LCI-4] (APC) and FITC conjugated mouse IgG1 isotype control. Figure B. ARG22952 anti-CD31 antibody [LCI-4] (APC) and FITC conjugated mouse anti porcine CD11R3. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
