ARG23474
anti-CD32 antibody [CCG36] (PE)
anti-CD32 antibody [CCG36] (PE) for Flow cytometry and Bovine,Sheep
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [CCG36] recognizes CD32 Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils. CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. Clone CCG36 also recognizes ovine CD32. |
|---|---|
| 反应物种 | Bov, Sheep |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CCG36 |
| 同位型 | IgG1 |
| 靶点名称 | CD32 |
| 抗原物种 | Bovine |
| 抗原 | Bovine FcγRII-transfected COS7 cells. |
| 偶联标记 | PE |
| 別名 | Fc-gamma-RIIc; Fc-gamma RII-c; CD32; CD antigen CD32; CD32B; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-c; FCG2; IGFR2; FcRII-c; FCGR2; CDw32 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q28110 Bovine Low affinity immunoglobulin gamma Fc region receptor II |
|---|---|
| 基因名称 | FCGR2B |
| 全名 | Fc fragment of IgG, low affinity IIb, receptor (CD32) |
| 背景介绍 | The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| 生物功能 | Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. [UniProt] |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Phosphorylated by the SRC-type Tyr-kinases LYN and BLK. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Bovine peripheral blood lymphocytes stained with ARG23474 anti-CD32 antibody [CCG36] (PE).
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Mouse anti Bovine CD282 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Bovine CD282 and ARG23474 anti-CD32 antibody [CCG36] (PE). All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
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ARG23474 anti-CD32 antibody [CCG36] (PE) FACS image
Flow Cytometry: Figure A. ARG23474 anti-CD32 antibody [CCG36] (PE) and FITC conjugated HuCAL isotype control. Figure B. ARG23474 anti-CD32 antibody [CCG36] (PE) and FITC conjugated Mouse anti Bovine CD282. All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
