ARG23533
anti-CD321 / JAM1 antibody [H202-106]
anti-CD321 / JAM1 antibody [H202-106] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse
概述
| 产品描述 | Rat Monoclonal antibody [H202-106] recognizes CD321 / JAM1. Rat anti Mouse CD321 antibody, clone H202-106 recognizes murine CD321, which is also known as junctional adhesion molecule 1 (JAM-1). CD321 is a 32-41kD glycoprotein, which shares similarities with related proteins JAM-2 and JAM-3. CD321 is a multifunctional protein that is primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localises with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability. In addition, CD321 is a ligand for the integrin LFA-1 and is also involved in the transmigration of leucocytes. |
|---|---|
| 反应物种 | Ms |
| 应用 | FACS, IHC-Fr, IP |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | H202-106 |
| 同位型 | IgG1 |
| 靶点名称 | CD321 / JAM1 |
| 抗原物种 | Mouse |
| 抗原 | MTE1/MTE2 stromal cell lines. |
| 偶联标记 | Un-conjugated |
| 別名 | JAM-1; JAM1; Junctional adhesion molecule A; CD antigen CD321; JCAM; JAM-A; Junctional adhesion molecule 1; PAM-1; JAM; JAMA; KAT; Platelet F11 receptor; Platelet adhesion molecule 1; CD321 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | F11R |
| 全名 | F11 receptor |
| 背景介绍 | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008] |
| 生物功能 | Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. [UniProt] |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG23533 anti-CD321 / JAM1 antibody [H202-106] FACS image
Flow Cytometry: Mouse peripheral blood platelets stained with ARG23533 anti-CD321 / JAM1 antibody [H202-106].
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ARG23533 anti-CD321 / JAM1 antibody [H202-106] FACS image
Flow Cytometry: Mouse peripheral blood platelets stained with ARG23533 anti-CD321 / JAM1 antibody [H202-106].
