ARG22956
anti-CD34 antibody [MEC14.7]
anti-CD34 antibody [MEC14.7] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
概述
| 产品描述 | Rat Monoclonal antibody [MEC14.7] recognizes CD34 Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies. |
|---|---|
| 反应物种 | Ms |
| 应用 | CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | MEC14.7 |
| 同位型 | IgG2a |
| 靶点名称 | CD34 |
| 抗原物种 | Mouse |
| 抗原 | T-end.1, a pMT transformed endothelial cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | Hematopoietic progenitor cell antigen CD34; CD antigen CD34 |
应用说明
| 应用建议 |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34 |
|---|---|
| 基因名称 | Cd34 |
| 全名 | CD34 antigen |
| 背景介绍 | CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| 生物功能 | CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt] |
| 产品亮点 | Related products: CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody |
| 预测分子量 | 41 kDa |
| 翻译后修饰 | Highly glycosylated. Phosphorylated on serine residues by PKC. |
检测图片 (1) Click the Picture to Zoom In
-
ARG22956 anti-CD34 antibody [MEC14.7] FACS image
Flow Cytometry: WEHI cells stained with ARG22956 anti-CD34 antibody [MEC14.7].
