ARG52756

anti-CD34 antibody [QBEND/10]

anti-CD34 antibody [QBEND/10] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Primates,Rabbit

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
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publication_link 参考文献2

概述

产品描述 Mouse Monoclonal antibody [QBEND/10] recognizes CD34 (Endothelial Cell Marker)
反应物种 Hu, Ms, NHuPrm, Rb
不反应物种 Rat, Bov, Dog, Sheep
应用 FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性 The antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND/10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. The clone QBEND/10 recognises the Class II epitope on CD34.
宿主 Mouse
克隆 Monoclonal
克隆号 QBEND/10
同位型 IgG1, kappa
靶点名称 CD34
抗原物种 Human
抗原 Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta.
偶联标记 Un-conjugated
別名 Hematopoietic progenitor cell antigen CD34; CD antigen CD34

应用说明

应用建议
应用 推荐稀释比
FACSAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:200
IPAssay-Dependent
WBAssay-dependent
应用说明 IHC-P:
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Lung, Tonsil
实际分子量 105-120 kDa

属性

形式 Liquid
缓冲液 Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide
抗菌剂 < 0.1% Sodium azide
稳定剂 1% BSA
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 12490 Mouse CD34

GeneID: 947 Human CD34

Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34

Swiss-port # Q64314 Mouse Hematopoietic progenitor cell antigen CD34

基因名称 CD34
全名 CD34 molecule
背景介绍 CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
生物功能 CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
细胞定位 Cytoplasm, Membrane
产品亮点 Related Antibody Duos and Panels:
ARG30300 Endothelial Cell Marker Antibody Duo (CD31, CD34)
ARG30328 Angiogenesis Antibody Panel
Related products:
CD34 antibodies; CD34 ELISA Kits; CD34 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Vascular development is regulated by FGF-dependent metabolic control
研究领域 Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
预测分子量 41 kDa
翻译后修饰 Highly glycosylated.
Phosphorylated on serine residues by PKC.

检测图片 (7) Click the Picture to Zoom In

  • ARG52756 anti-CD34 antibody [QBEND/10] ICC/IF image

    Immunofluorescence: hCMEC/D3 cells stained with ARG52756 anti-CD34 antibody [QBEND/10].

    From Lucie Y Li et al. Front Cell Neurosci. (2023), doi: 10.3389/fncel.2023.1077204, Fig. 3A & B.

  • ARG52756 anti-CD34 antibody [QBEND/10] WB image

    Western blot: 30 µg of mouse lung lysate stained with ARG52756 anti-CD34 antibody [QBEND/10] at 1:500 dilution.

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Breast Ductal Carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Cervical Squamous Cell carcinoma stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Colon stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Placenta stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

  • ARG52756 anti-CD34 antibody [QBEND/10] IHC-P image

    Immunohistochemistry: Human Tonsil stained with CD34 (Endothelial Cell Marker) antibody [QBEnd/10] (ARG52756)

客户反馈

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anti-CD34 antibody [QBEND/10]

Application:WB

Sample:Mouse lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Brain blood vessel autoantibodies in patients with NMDA and GABAA receptor encephalitis: identification of unconventional Myosin-X as target antigen

ICC/IF / Human

Lucie Y Li et al.
Front Cell Neurosci.,  (2023)

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Role of Angiogenesis in Chronic Radiation Proctitis: New Evidence Favoring Inhibition of Angiogenesis Ex Vivo.

IHC-P / Human

Wu Pei-Huang et al.
Dig Dis Sci.,  (2017)

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