ARG52768
anti-CD4 antibody [SP35]
anti-CD4 antibody [SP35] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
概述
| 产品描述 | Rabbit Monoclonal antibody [SP35] recognizes CD4 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Pig |
| 应用 | FACS, IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SP35 |
| 同位型 | IgG |
| 靶点名称 | CD4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to internal region of human CD4. |
| 偶联标记 | Un-conjugated |
| 別名 | CD4mut; CD antigen CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM EDTA buffer for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | T Cell Lymphoma, Tonsil |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by protein A/G |
| 缓冲液 | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| 抗菌剂 | < 0.1% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD4 |
| 全名 | CD4 molecule |
| 背景介绍 | CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010] |
| 生物功能 | CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. [UniProt] |
| 细胞定位 | Membrane |
| 产品亮点 | Related Antibody Duos and Panels: ARG30086 Regulatory T cells Marker Antibody Duo (FoxP3, CD4) Related products: CD4 antibodies; CD4 ELISA Kits; CD4 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Regulatory T cells Study antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. |
检测图片 (3) Click the Picture to Zoom In
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ARG52768 anti-CD4 antibody [SP35] Flow Cytometry image
Flow Cytometry: Jurkat cells stained with ARG52768 anti-CD4 antibody [SP35] (green) and control IgG (blue).
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ARG52768 anti-CD4 antibody [SP35] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD4 antibody [SP35] (ARG52768)
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ARG52768 anti-CD4 antibody [SP35] IHC-P image
Immunohistochemistry: Human Tonsil stained with CD4 antibody [SP35] (ARG52768)
