ARG22945
anti-CD40 antibody [LOB7/6] (PE)
anti-CD40 antibody [LOB7/6] (PE) for Flow cytometry and Human,Dog
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [LOB7/6] recognizes CD40 Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.CD40 is involved in the process of B cell selection in germinal centres and is vital in T cell-B cell interactions. |
|---|---|
| 反应物种 | Hu, Dog |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LOB7/6 |
| 同位型 | IgG2a |
| 靶点名称 | CD40 |
| 抗原物种 | Human |
| 抗原 | CD40 Ig(Fc) fusion protein containing the EC region of Human CD40 and Fc region of Human IgG. |
| 偶联标记 | PE |
| 別名 | CDw40; CD antigen CD40; Tumor necrosis factor receptor superfamily member 5; Bp50; CD40L receptor; CDW40; TNFRSF5; p50; B-cell surface antigen CD40 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Unpurified. |
| 缓冲液 | PBS, 0.09% Sodium azide and 1% BSA |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P25942 Human Tumor necrosis factor receptor superfamily member 5 |
|---|---|
| 基因名称 | CD40 |
| 全名 | CD40 molecule, TNF receptor superfamily member 5 |
| 背景介绍 | This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014] |
| 生物功能 | Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Pro-B Cell Marker antibody; Pre-B Cell Marker antibody |
| 预测分子量 | 31 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG22945 anti-CD40 antibody [LOB7/6] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Mouse anti Human CD19 and PE-conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Human CD19 and ARG22945 anti-CD40 antibody [LOB7/6] (PE). All experiments performed on red cell lysed Human peripheral blood gated on lymphocytes.
