ARG62846

anti-CD42b antibody [HIP1]

Datasheet

订购信息

包装

价格 (USD)

100 μg

399

arigo in CHINA:
恩瑞科生物
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概述

产品描述	Mouse Monoclonal antibody [HIP1] recognizes CD42b
反应物种	Hu, NHuPrm
应用	CyTOF®-candidate, FACS, FuncSt, IHC-Fr
特异性	The clone HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40
宿主	Mouse
克隆	Monoclonal
克隆号	HIP1
同位型	IgG1
靶点名称	CD42b
抗原	Peripheral blood mononuclear cells of a patient suffering with CLL.
偶联标记	Un-conjugated
別名	CD antigen CD42b; Antigen CD42b-alpha; DBPLT3; VWDP; CD42B; GP-Ib alpha; Glycoprotein Ibalpha; BDPLT1; BSS; CD42b-alpha; GPIbA; GPIb-alpha; Platelet glycoprotein Ib alpha chain; BDPLT3; GP1B

应用说明

应用建议

应用	推荐稀释比
CyTOF®-candidate	Assay-dependent
FACS	1 - 4 µg/ml
FuncSt	Assay-dependent
IHC-Fr	Assay-dependent

应用说明

Functional studies: The clone HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified by protein A
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2811 Human GP1BA Swiss-port # P07359 Human Platelet glycoprotein Ib alpha chain
基因名称	GP1BA
全名	glycoprotein Ib (platelet), alpha polypeptide
背景介绍	CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
生物功能	GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. [UniProt]
产品亮点	Related products: CD42b antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies
研究领域	Cell Biology and Cellular Response antibody; Immune System antibody
预测分子量	72 kDa
翻译后修饰	Glycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis.

检测图片 (2) Click the Picture to Zoom In

ARG62846 anti-CD42b antibody [HIP1] FACS image

Flow Cytometry: Human peripheral blood cells stained with ARG62846 anti-CD42b antibody [HIP1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG62846 anti-CD42b antibody [HIP1] FACS image

Flow Cytometry: Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62846 anti-CD42b antibody [HIP1] at 4 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.