ARG62850

anti-CD44 antibody [MEM-85] (Biotin)

anti-CD44 antibody [MEM-85] (Biotin) for Flow cytometry and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody

概述

产品描述 Biotin-conjugated Mouse Monoclonal antibody [MEM-85] recognizes CD44
反应物种 Hu
应用 FACS
特异性 The clone MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
HLDA IV; WS Code NL 706
HLDA VI; WS Code AS Ref.15
宿主 Mouse
克隆 Monoclonal
克隆号 MEM-85
同位型 IgG2b
靶点名称 CD44
抗原 Leukocytes of a patient suffering from LGL Type Leukaemia.
偶联标记 Biotin
別名 MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化说明 The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
缓冲液 PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂 15 mM Sodium azide
浓度 1 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 960 Human CD44

Swiss-port # P16070 Human CD44 antigen

基因名称 CD44
全名 CD44 molecule (Indian blood group)
背景介绍 CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
生物功能 Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. [UniProt]
研究领域 Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
预测分子量 82 kDa
翻译后修饰 Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

检测图片 (1) Click the Picture to Zoom In

  • ARG62850 anti-CD44 antibody [MEM-85] (Biotin) FACS image

    Flow Cytometry: Human peripheral blood leukocytes stained with ARG62850 anti-CD44 antibody [MEM-85] (Biotin), followed by Streptavidin (APC).

克隆号文献

Umbilical cord mesenchymal stem cells suppress host rejection: the role of the glycocalyx.

ICC/IF / Human

Coulson-Thomas VJ et al.
J Biol Chem.,  (2014)

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Organization and regulation of intracellular plasma membrane-connected HIV-1 assembly compartments in macrophages.

ICC/IF / Human

Mlcochova P et al.
BMC Biol.,  (2013)

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Human leucocyte surface glycoprotein CDw44 and lymphocyte homing receptor are identical molecules.

Stefanová I et al.
Immunogenetics.,  (1989)

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Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Bazil V et al.
Folia Biol (Praha).,  (1989)

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