ARG23141
anti-CD49c / Integrin alpha 3 antibody [17C6] (PE)
anti-CD49c / Integrin alpha 3 antibody [17C6] (PE) for Flow cytometry and Human
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [17C6] recognizes CD49c / Integrin alpha 3 Mouse anti Human CD49c antibody, clone 17C6 recognizes CD49c, a 150kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell cell line. |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 17C6 |
| 同位型 | IgG1 |
| 靶点名称 | CD49c / Integrin alpha 3 |
| 抗原物种 | Human |
| 抗原 | CD49c transfected cell line. |
| 偶联标记 | PE |
| 別名 | FRP-2; MSK18; GAPB3; CD49 antigen-like family member C; CD49C; ILNEB; Integrin alpha-3; VL3A; Galactoprotein B3; VLA3a; CD antigen CD49c; VCA-2; VLA-3 subunit alpha; GAP-B3 |
应用说明
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGA3 |
| 全名 | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| 背景介绍 | The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| 生物功能 | Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. [UniProt] |
| 预测分子量 | 117 kDa |
| 翻译后修饰 | Isoform 1, but not isoform 2, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG23141 anti-CD49c / Integrin alpha 3 antibody [17C6] (PE) FACS image
Flow Cytometry: KG1 cell line stained with ARG23141 anti-CD49c / Integrin alpha 3 antibody [17C6] (PE).
