ARG66228
anti-CD49c / Integrin alpha 3 antibody [ASC-1]
anti-CD49c / Integrin alpha 3 antibody [ASC-1] for CyTOF®-candidate,Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [ASC-1] recognizes CD49c / Integrin alpha 3 |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, IHC-Fr, IP, WB |
| 特异性 | This antibody recognizes CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ASC-1 |
| 同位型 | IgG1 |
| 靶点名称 | CD49c / Integrin alpha 3 |
| 抗原物种 | Human |
| 抗原 | Human SSC-9 cell line (squamous cell carcinoma). |
| 偶联标记 | Un-conjugated |
| 別名 | FRP-2; MSK18; GAPB3; CD49 antigen-like family member C; CD49C; ILNEB; Integrin alpha-3; VL3A; Galactoprotein B3; VLA3a; CD antigen CD49c; VCA-2; VLA-3 subunit alpha; GAP-B3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide. |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGA3 |
| 全名 | integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) |
| 背景介绍 | The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
| 生物功能 | Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. [UniProt] |
| 产品亮点 | Related products: CD49c antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 预测分子量 | A transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains. |
| 翻译后修饰 | Isoform 1, but not isoform 2, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12-myristate 13-acetate stimulation. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] FACS image
Flow Cytometry: HeLa cells stained with ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1].
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ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] WB image
Western blot: Caco2 cells covered with matrigel for 0 or 7 days (Lane 1 & Lane 2) and Integrin alpha 3 overexpressing Caco2 cells. The blots were stained with ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1].
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ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] IP image
Immunoprecipitation: Biotinylated SCC9 cell extract were immunoprecipitated by control mouse IgG (Lane 1) or ARG66228 anti-CD49c / Integrin alpha 3 antibody [ASC-1] (Lane 2 & Lane 3). Then run SDS-PAGE at non-reducing condition (Lane 2) or reducing condition (Lane 3).
