ARG66751
anti-CD5 antibody [SQab20184]
anti-CD5 antibody [SQab20184] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20184] recognizes CD5 |
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| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20184 |
| 同位型 | IgG |
| 靶点名称 | CD5 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein of Human CD5 (extracellular). The immunogen contains the specific extracellular domain (D32-P372). |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD5; Lymphocyte antigen T1/Leu-1; LEU1; T-cell surface glycoprotein CD5; T1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WB: Jurkat IHC-P: Appendix tissue |
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| 实际分子量 | ~ 52 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD5 |
| 全名 | CD5 molecule |
| 背景介绍 | This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016] |
| 生物功能 | May act as a receptor in regulating T-cell proliferation. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66751 anti-CD5 antibody [SQab20184] WB image (Customer's Feedback)
Western blot: 10 µg of Jurkat cell lysate stained with ARG66751 anti-CD5 antibody [SQab20184] at 1:1000 dilution.
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ARG66751 anti-CD5 antibody [SQab20184] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human appendix tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66751 anti-CD5 antibody [SQab20184] at 18°C - 25°C for 30 minutes.
客户反馈
Excellent
anti-CD5 antibody [SQab20184]
Application：WB
Sample：Jurkat
Sample Loading Amount：10 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
