ARG22824

anti-CD52 antibody [YTH34.5] (low endotoxin)

anti-CD52 antibody [YTH34.5] (low endotoxin) for ELISA,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,IHC-Resin sections,Immunoprecipitation,Western blot and Human,Rhesus Monkey

概述

产品描述 Azide free and low endotoxin Rat Monoclonal antibody [YTH34.5] recognizes CD52
Rat anti Human CD52 antibody, clone YTH34.5 reacts with the human CD52 antigen, also known as CAMPATH-1. The CD52 antigen is a remarkably small but heavily glycosylated peptide attached to the cell surface membrane via a GPI link (Xia et al. 1991). The apparent molecular mass of the native antigen on SDS-PAGE is 25-29kDa, considerably reduced following N-glycanase treatment (Rowan et al. 1998).CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies (Dearden et al. 2002; Pettitt et al. 2012).
反应物种 Hu, R. Mk
应用 ELISA, FACS, IHC-Fr, IHC-P, IHC-R, IP, WB
宿主 Rat
克隆 Monoclonal
克隆号 YTH34.5
同位型 IgG2b
靶点名称 CD52
抗原物种 Human
抗原 Human lymphocytes.
偶联标记 Un-conjugated
別名 He5; CAMPATH-1 antigen; Human epididymis-specific protein 5; Cambridge pathology 1 antigen; CDw52; Epididymal secretory protein E5; CDW52; CD antigen CD52

应用说明

应用建议
应用 推荐稀释比
ELISAAssay-dependent
FACS1:20
IHC-Fr1:20
IHC-PAssay-dependent
IHC-RAssay-dependent
IPAssay-dependent
WBAssay-dependent
应用说明 FACS: use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A.
纯化说明 Low endotoxin
缓冲液 PBS
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 1043 Human CD52

Swiss-port # P31358 Human CAMPATH-1 antigen

基因名称 CD52
全名 CD52 molecule
生物功能 May play a role in carrying and orienting carbohydrate, as well as having a more specific role. [UniProt]
预测分子量 7 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG22824 anti-CD52 antibody [YTH34.5] (low endotoxin) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG22824 anti-CD52 antibody [YTH34.5] (low endotoxin).