ARG62889
anti-CD54 / ICAM1 antibody [1H4] (FITC)
anti-CD54 / ICAM1 antibody [1H4] (FITC) for Flow cytometry and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
概述
| 产品描述 | FITC-conjugated Mouse Monoclonal antibody [1H4] recognizes CD54 / ICAM1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1H4 |
| 同位型 | IgG2b |
| 靶点名称 | CD54 / ICAM1 |
| 抗原物种 | Human |
| 抗原 | Raji cells and spleen cells fused with NS1 cells |
| 偶联标记 | FITC |
| 別名 | CD54; CD antigen CD54; BB2; P3.58; Intercellular adhesion molecule 1; Major group rhinovirus receptor; ICAM-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ICAM1 |
| 全名 | intercellular adhesion molecule 1 |
| 背景介绍 | This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008] |
| 生物功能 | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30162 Cytotoxic T Cell Surface Marker Antibody Panel (FACS) Related products: CD54 antibodies; CD54 ELISA Kits; CD54 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody |
| 预测分子量 | 58 kDa |
| 翻译后修饰 | Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. |
检测图片 (1) Click the Picture to Zoom In
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ARG62889 anti-CD54 / ICAM1 antibody [1H4] (FITC) FACS image
Flow Cytometry: Separation of Human CD54 positive Monocytes (red) from Human CD54 negative Lymphocytes (black-dashed). Human peripheral blood stained with ARG62889 anti-CD54 / ICAM1 antibody [1H4] (FITC).
克隆号文献
Dendritic cell-lymphocyte cross talk downregulates host restriction factor SAMHD1 and stimulates HIV-1 replication in dendritic cells.
Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.
Stimulation of HIV-1 replication in immature dendritic cells in contact with primary CD4 T or B lymphocytes.
FACS / Human
Regulation of expression of a human intercellular adhesion molecule (ICAM-1) during lymphohematopoietic differentiation.
ICC/IF / Human
