ARG62894
anti-CD56 / NCAM antibody [MEM-188]
anti-CD56 / NCAM antibody [MEM-188] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human,Primates
Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-188] recognizes CD56 / NCAM |
|---|---|
| 反应物种 | Hu, NHuPrm |
| 应用 | FACS, ICC/IF, IHC-P, IP |
| 特异性 | The clone MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested. HLDA VI; WS code A055 HLDA VI; WS Code NK26 HLDA VII; WS code 70077 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-188 |
| 同位型 | IgG2a |
| 靶点名称 | CD56 / NCAM |
| 抗原物种 | Human |
| 抗原 | KG-1 human acute myelogenous leukemia cell line |
| 偶联标记 | Un-conjugated |
| 別名 | CD56; NCAM; CD antigen CD56; N-CAM-1; MSK39; NCAM-1; Neural cell adhesion molecule 1 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Pretreatment: Heat retrieval (microwave oven) in citrate buffer, standard ABC visualization. Staining technique: Standard ABC technique (DAB+). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | IHC-P: Positive membrane-specific reaction in adjacent peripheral nerve staining of paraprostatic autonomic ganglion. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NCAM1 |
| 全名 | neural cell adhesion molecule 1 |
| 背景介绍 | CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of immunoglobulin family serving as adhesive molecule which is ubiquitously expressed in nervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on T cells and NK cells. Polysialic modification results in reduction of CD56-mediated cell adhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumours and ovarian granulosa cell tumours. |
| 生物功能 | This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. [UniProt] |
| 产品亮点 | Related products: CD56 antibodies; CD56 ELISA Kits; CD56 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma Tumor-Infiltrating Lymphocytes (TILs) |
| 研究领域 | Developmental Biology antibody; Neuroscience antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody |
| 预测分子量 | 95 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG62894 anti-CD56 / NCAM antibody [MEM-188] FACS image
Flow Cytometry: Human peripheral blood lymphocytes stained with ARG62894 anti-CD56 / NCAM antibody [MEM-188], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.
克隆号文献
Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.
FACS / Mouse
