ARG40201
anti-CD61 / Integrin beta 3 antibody
anti-CD61 / Integrin beta 3 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD61 / Integrin beta 3 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Hm |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD61 / Integrin beta 3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 752-770 of Human CD61 / Integrin beta 3. (EFAKFEEERARAKWDTANN) |
| 偶联标记 | Un-conjugated |
| 別名 | GT; CD antigen CD61; CD61; BDPLT2; GPIIIa; BDPLT16; GP3A; Platelet membrane glycoprotein IIIa; Integrin beta-3 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB3 |
| 全名 | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| 背景介绍 | The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008] |
| 生物功能 | Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane. Cell junction, focal adhesion. [UniProt] |
| 预测分子量 | 87 kDa |
| 翻译后修饰 | Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40201 anti-CD61 / Integrin beta 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG40201 anti-CD61 / Integrin beta 3 antibody.
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ARG40201 anti-CD61 / Integrin beta 3 antibody WB image
Western blot: Jurkat, Raji, CEM and COLO320 cell lysates stained with ARG40201 anti-CD61 / Integrin beta 3 antibody.
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ARG40201 anti-CD61 / Integrin beta 3 antibody FACS image
Flow Cytometry: Human PBMC cells were blocked with 10% normal goat serum and then stained with ARG40201 anti-CD61 / Integrin beta 3 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
