ARG62905
anti-CD62L / L-Selectin antibody [DREG56]
anti-CD62L / L-Selectin antibody [DREG56] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, FuncSt, IHC-Fr, IP, WB |
| 特异性 | The clone DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V; WS Code S056 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | DREG56 |
| 同位型 | IgG1 |
| 靶点名称 | CD62L / L-Selectin |
| 抗原物种 | Human |
| 抗原 | PMA-activated human peripheral blood leukocytes |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
应用说明
| 应用建议 |
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| 应用说明 | WB: Under non-reducing condition. Functional studies: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SELL |
| 全名 | selectin L |
| 背景介绍 | CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells. |
| 生物功能 | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt] |
| 产品亮点 | Related products: CD62L antibodies; CD62L ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 42 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG62905 anti-CD62L / L-Selectin antibody [DREG56] FACS image
Flow Cytometry: Human peripheral blood stained with ARG62905 anti-CD62L / L-Selectin antibody [DREG56] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG62905 anti-CD62L / L-Selectin antibody [DREG56] FACS image
Flow Cytometry: Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62905 anti-CD62L / L-Selectin antibody [DREG56] at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
克隆号文献
In Vitro and in Vivo Characterization of Molecular Interactions between Calmodulin, Ezrin/Radixin/Moesin, and L-selectin.
Critical role of Lck in L-selectin signaling induced by sulfatides engagement.
L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts.
Selectin blockade prevents antigen-induced late bronchial responses and airway hyperresponsiveness in allergic sheep.
