ARG42260
anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin)
anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Immunoprecipitation,Western blot and Human
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [DREG56] recognizes CD62L / L-Selectin |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-Fr, IP, WB |
| 特异性 | The mouse monoclonal antibody DREG56 recognizes an extracellular epitope of CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | DREG56 |
| 同位型 | IgG1 |
| 靶点名称 | CD62L / L-Selectin |
| 抗原物种 | Human |
| 抗原 | PMA-activated Human peripheral blood leukocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.01 EU/µg of the protein. |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SELL |
| 全名 | selectin L |
| 背景介绍 | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| 生物功能 | Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641). Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641). [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 42 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
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ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) FACS image
Flow Cytometry: Separation of Human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG42260 anti-CD62L / L-Selectin antibody [DREG56] (low endotoxin) at 1 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
