ARG57664
anti-CD63 antibody
anti-CD63 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey
概述
| 产品描述 | Goat Polyclonal antibody recognizes CD63 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Dog, Mk |
| 应用 | ICC/IF, WB |
| 特异性 | The antibody reacts with CD63, a 40-60 kDa glycoprotein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD63 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around aa. 120-175 of Human CD63. |
| 偶联标记 | Un-conjugated |
| 別名 | Tspan-30; CD63 antigen; Tetraspanin-30; CD antigen CD63; Lysosomal-associated membrane protein 3; OMA81H; Ocular melanoma-associated antigen; Granulophysin; TSPAN30; Melanoma-associated antigen ME491; MLA1; LAMP-3; ME491 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Human (HeLa, HUH, Jurkat), Mouse (AtT-20, Hepa, 3T3, RAW264.7), Canine (MDCK) and Monkey (COS-7) whole cell lysates. | ||||||
| 实际分子量 | 40 - 60 kDa (glycoprotein) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.05% Sodium azide and 20% Glycerol. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 20% Glycerol |
| 浓度 | 3 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD63 |
| 全名 | CD63 molecule |
| 背景介绍 | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| 生物功能 | Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli. [UniProt] |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG57664 anti-CD63 antibody ICC/IF image
Immunofluorescence: 4% PFA fixed Hepa1-6 cells stained with ARG57664 anti-CD63 antibody at 1:50 dilution.
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ARG57664 anti-CD63 antibody WB image
Western blot: 50 µg of Huh cell lysate stained with ARG57664 anti-CD63 antibody at 1:2500 dilution.
