ARG66809
anti-CD66e / CEA antibody [SQab20222]
anti-CD66e / CEA antibody [SQab20222] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20222] recognizes CD66e / CEA |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20222 |
| 同位型 | IgG |
| 靶点名称 | CD66e / CEA |
| 抗原物种 | Human |
| 抗原 | Full length Human CD66e / CEA protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; CD antigen CD66e; Carcinoembryonic antigen; Meconium antigen 100 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | 180 - 200 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P06731 Human Carcinoembryonic antigen-related cell adhesion molecule 5 |
|---|---|
| 基因名称 | CEACAM5 |
| 全名 | carcinoembryonic antigen-related cell adhesion molecule 5 |
| 背景介绍 | This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| 生物功能 | Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:2803308, PubMed:10910050, PubMed:10864933). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050). (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66809 anti-CD66e / CEA antibody [SQab20222] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human colon carcinoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66809 anti-CD66e / CEA antibody [SQab20222].
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ARG66809 anti-CD66e / CEA antibody [SQab20222] WB image (Customer review)
Western blot: HT29 stained with ARG66809 anti-CD66e / CEA antibody [SQab20222] at 1:500 dilution.
客户反馈
Excellent
anti-CD66e / CEA antibody [SQab20222]
Application：WB
Sample：HT29
Sample Loading Amount：10 μL
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
