ARG62916
anti-CD69 antibody [FN50]
anti-CD69 antibody [FN50] for CyTOF®-candidate,Flow cytometry,Functional study,IHC-Frozen sections and Human
Developmental Biology antibody; Immune System antibody
概述
| 产品描述 | Mouse Monoclonal antibody [FN50] recognizes CD69 |
|---|---|
| 反应物种 | Hu |
| 应用 | CyTOF®-candidate, FACS, FuncSt, IHC-Fr |
| 特异性 | The clone FN50 recognizes CD69, an lymphocyte early activation marker. HLDA IV; WS Code A 91 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | FN50 |
| 同位型 | IgG1 |
| 靶点名称 | CD69 |
| 抗原物种 | Human |
| 抗原 | anti-µ-stimulated human B lymphocytes |
| 偶联标记 | Un-conjugated |
| 別名 | GP32/28; Activation inducer molecule; MLR-3; BL-AC/P26; Leukocyte surface antigen Leu-23; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; EA1; CD antigen CD69; CLEC2C; C-type lectin domain family 2 member C |
应用说明
| 应用建议 |
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| 应用说明 | Functional studies: When stimulating peripheral blood T cells, clone FN50 together with TPA synergistically increases cell volume and RNA/DNA synthesis. Addition of FN50 to stimulated peripheral blood B cells has similar, but weaker effect. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from cell culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD69 |
| 全名 | CD69 molecule |
| 背景介绍 | CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets; it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy. |
| 生物功能 | Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. [UniProt] |
| 产品亮点 | Related products: CD69 antibodies; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 23 kDa |
| 翻译后修饰 | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
检测图片 (1) Click the Picture to Zoom In
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ARG62916 anti-CD69 antibody [FN50] FACS image
Flow Cytometry: Human PHA-activated peripheral blood stained with ARG62916 anti-CD69 antibody [FN50], followed by incubation with APC labelled Goat anti-Mouse secondary antibody.
克隆号文献
CD98hc (SLC3A2) interaction with beta 1 integrins is required for transformation.
Multiple dimeric forms of human CD69 result from differential addition of N-glycans to typical (Asn-X-Ser/Thr) and atypical (Asn-X-cys) glycosylation motifs.
