ARG41865
anti-CD71 / Transferrin Receptor antibody
anti-CD71 / Transferrin Receptor antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD71 / Transferrin Receptor |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD71 / Transferrin Receptor |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-140 of Human CD71 / Transferrin Receptor. (NP_001121620.1) |
| 偶联标记 | Un-conjugated |
| 別名 | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse liver | ||||||||
| 实际分子量 | ~ 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TFRC |
| 全名 | transferrin receptor |
| 背景介绍 | This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type II membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Transferrin receptor protein 1, serum form: Secreted. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. [UniProt] |
检测图片 (4) Click the Picture to Zoom In
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ARG41865 anti-CD71 / Transferrin Receptor antibody ICC/IF image
Immunofluorescence: L929 cells stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:100 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue stained with ARG41865 anti-CD71 / Transferrin Receptor antibody 1:100 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody WB image
Western blot: 25 µg of Mouse liver lysate stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:3000 dilution.
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ARG41865 anti-CD71 / Transferrin Receptor antibody IP image
Immunoprecipitation: 200 µg extracts of Jurkat cells were immunoprecipitated and stained with ARG41865 anti-CD71 / Transferrin Receptor antibody at 1:1000 dilution.
