ARG55075
anti-CD73 antibody [974CT7.5.3]
anti-CD73 antibody [974CT7.5.3] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
概述
| 产品描述 | Mouse Monoclonal antibody recognizes CD73 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 974CT7.5.3 |
| 同位型 | IgG1 |
| 靶点名称 | CD73 |
| 抗原物种 | Human |
| 抗原 | Purified His-tagged Human CD73 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 阳性对照 | WiDr |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | Crude Ascites and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NT5E |
| 全名 | 5'-nucleotidase, ecto (CD73) |
| 背景介绍 | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011] |
| 生物功能 | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 63 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG55075 anti-CD73 antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human kidney blocked with 3% BSA for 30 min at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG55075 anti-CD73 antibody at 1:25 for 1 hours at 37°C.
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ARG55075 anti-CD73 antibody WB image
Western blot: 20 µg of WiDr whole cell lysate stained with ARG55075 anti-CD73 antibody at 1:2000 dilution.
