ARG41737

anti-CD73 antibody

anti-CD73 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes CD73
反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 CD73
抗原物种 Human
抗原 Recombinant fusion protein corresponding to aa. 290-550 of Human CD73 (NP_002517.1).
偶联标记 Un-conjugated
別名 5'-nucleotidase; CD antigen CD73; eN; CALJA; CD73; EC 3.1.3.5; NT; Ecto-5'-nucleotidase; NT5; E5NT; 5'-NT; eNT; NTE

应用说明

应用建议
应用 推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照 Mouse lung
实际分子量 ~ 73 kDa

属性

形式 Liquid
纯化 Affinity purified.
缓冲液 PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 23959 Mouse NT5E

GeneID: 4907 Human NT5E

Swiss-port # P21589 Human 5'-nucleotidase

Swiss-port # Q61503 Mouse 5'-nucleotidase

基因名称 NT5E
全名 5'-nucleotidase, ecto (CD73)
背景介绍 The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
生物功能 Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. [UniProt]
细胞定位 Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
预测分子量 63 kDa

检测图片 (2) Click the Picture to Zoom In

  • ARG41737 anti-CD73 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG41737 anti-CD73 antibody at 1:200 dilution.

  • ARG41737 anti-CD73 antibody WB image

    Western blot: 25 µg of Mouse lung lysate stained with ARG41737 anti-CD73 antibody at 1:1000 dilution.