ARG41757
anti-CD8 / CD8 alpha antibody
anti-CD8 / CD8 alpha antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes CD8 / CD8 alpha |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD8 / CD8 alpha |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide of Human CD8 / CD8 alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | T-cell surface glycoprotein CD8 alpha chain; Leu2; p32; T-lymphocyte differentiation antigen T8/Leu-2; CD8; MAL; CD antigen CD8a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Jurkat | ||||||||
| 实际分子量 | ~ 32 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P01732 Human T-cell surface glycoprotein CD8 alpha chain |
|---|---|
| 基因名称 | CD8A |
| 全名 | CD8a molecule |
| 背景介绍 | CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| 生物功能 | CD8 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. [UniProt] |
| 细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=CD8A localizes to lipid rafts only when associated with its partner CD8B. Isoform 2: Secreted. [UniProt] |
| 产品亮点 | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Detecting exosomal HMGB1 for ICD research |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Cytotoxic T antibody; Cytotoxic T Cell Surface Study antibody; Tumor-infiltrating Lymphocyte Study antibody |
| 预测分子量 | 26 kDa |
| 翻译后修饰 | All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG41757 anti-CD8 / CD8 alpha antibody ICC/IF image
Immunofluorescence: Jurkat cells stained with ARG41757 anti-CD8 / CD8 alpha antibody (green). Nuclear staining (blue).
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ARG41757 anti-CD8 / CD8 alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG41757 anti-CD8 / CD8 alpha antibody.
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ARG41757 anti-CD8 / CD8 alpha antibody WB image
Western blot: Jurkat cell lysate stained with ARG41757 anti-CD8 / CD8 alpha antibody.
